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cropseq multi v2 mtagbfp2 nls plasmid  (Addgene inc)


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    Structured Review

    Addgene inc cropseq multi v2 mtagbfp2 nls plasmid
    Cropseq Multi V2 Mtagbfp2 Nls Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cropseq+multi+v2+mtagbfp2+nls+plasmid/Flag-HA-USP22+(Plasmid+%2322575)/pmc12545207-251-7-9
    Average 93 stars, based on 4 article reviews
    cropseq multi v2 mtagbfp2 nls plasmid - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Systematic discovery of CRISPR-boosted CAR T cell immunotherapies
    Article Snippet: The pPD0035_CROP-seq-CAR-Puro_CD19-28z (Addgene, 242983) vector was generated by replacing the sequence for the intracellular 4-1BB co-stimulatory domain with the sequence for the CD28 co-stimulatory domain ordered as a gBlock (IDT). .. The CROP-seq-CAR-multi vectors were cloned from the CROPseq-multi-v2-mTagBFP2-NLS plasmid (Addgene, 225754), which was amplified with primers CROPseq_FWD_016-minusACC and CROPseq-multi-v2_REV1 (Supplementary Table ) using Q5 High-Fidelity 2X Master Mix (NEB, M0492L) to generate the backbone for all variants of the CROP-seq-CAR-multi vectors (7,622 bp, including 40-bp extensions), followed by DpnI digestion (NEB, R0176L) to remove the parental plasmid. ..

    Plasmid Preparation:

    Article Title: Systematic discovery of CRISPR-boosted CAR T cell immunotherapies
    Article Snippet: The pPD0035_CROP-seq-CAR-Puro_CD19-28z (Addgene, 242983) vector was generated by replacing the sequence for the intracellular 4-1BB co-stimulatory domain with the sequence for the CD28 co-stimulatory domain ordered as a gBlock (IDT). .. The CROP-seq-CAR-multi vectors were cloned from the CROPseq-multi-v2-mTagBFP2-NLS plasmid (Addgene, 225754), which was amplified with primers CROPseq_FWD_016-minusACC and CROPseq-multi-v2_REV1 (Supplementary Table ) using Q5 High-Fidelity 2X Master Mix (NEB, M0492L) to generate the backbone for all variants of the CROP-seq-CAR-multi vectors (7,622 bp, including 40-bp extensions), followed by DpnI digestion (NEB, R0176L) to remove the parental plasmid. ..

    Amplification:

    Article Title: Systematic discovery of CRISPR-boosted CAR T cell immunotherapies
    Article Snippet: The pPD0035_CROP-seq-CAR-Puro_CD19-28z (Addgene, 242983) vector was generated by replacing the sequence for the intracellular 4-1BB co-stimulatory domain with the sequence for the CD28 co-stimulatory domain ordered as a gBlock (IDT). .. The CROP-seq-CAR-multi vectors were cloned from the CROPseq-multi-v2-mTagBFP2-NLS plasmid (Addgene, 225754), which was amplified with primers CROPseq_FWD_016-minusACC and CROPseq-multi-v2_REV1 (Supplementary Table ) using Q5 High-Fidelity 2X Master Mix (NEB, M0492L) to generate the backbone for all variants of the CROP-seq-CAR-multi vectors (7,622 bp, including 40-bp extensions), followed by DpnI digestion (NEB, R0176L) to remove the parental plasmid. ..



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    cropseq multi v2 mtagbfp2 nls plasmid  (Addgene inc)
    93
    Addgene inc cropseq multi v2 mtagbfp2 nls plasmid
    Cropseq Multi V2 Mtagbfp2 Nls Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cropseq+multi+v2+mtagbfp2+nls+plasmid/Flag-HA-USP22+(Plasmid+%2322575)/pmc12545207-251-7-9
    Average 93 stars, based on 1 article reviews
    cropseq multi v2 mtagbfp2 nls plasmid - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

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